Light tailored by multimode fiber for multiphoton fluorescence microscopy

TL;DR

This paper demonstrates how to generate specialized light beams using multimode fibers to enhance axial resolution in two-photon fluorescence microscopy, offering a simple and cost-effective approach.

Contribution

It introduces a novel method of producing azimuthally invariant guided mode beams via multimode fibers for improved microscopy resolution.

Findings

Beams can be generated by injecting Gaussian light into multimode fibers.

The method enhances axial resolution in two-photon microscopy.

The approach is low-cost and minimally complex.

Abstract

We study the diffraction of a particular class of beams, composed only by a combination of azimuthally invariant guided modes of an optical fiber. We demonstrate that such beams can be obtained by injecting a Gaussian beam in a small piece of silica graded-index multimode fiber. This minimalistic low-cost method is applied for improving the axial resolution of a two-photon microscope.