Treatment of seeds by cold ambient air plasma: Combining impedance measurements with water sorption modeling to understand the impact of seed hydration

TL;DR

This study investigates how cold ambient air plasma affects Arabidopsis seeds with different water contents, revealing changes in dielectric properties and water affinity that could influence seed hydration and plasma interactions.

Contribution

It combines impedance measurements with water sorption modeling to understand seed-plasma interactions and shows plasma primarily enhances water-seed affinity without altering seed composition.

Findings

Increased water content enhances plasma streamer activity.

Plasma exposure does not significantly change seed dielectric parameters.

Water affinity of seeds is strengthened by plasma through molecular interaction modifications.

Abstract

In this article, we focus on the plasma seed interaction and more specifically-on the feedback exerted by the seeds on the plasma properties. Dormant Arabidopsis seeds with different water contents (WC), namely 3 %DW, 10 %DW and 30%DW were exposed to cold ambient air plasma (C2AP) generated in a dielectric barrier device (DBD). It is found that increasing WC enhances the capacitive current of the DBD, generates a greater number of low energy streamers (characterized by current peaks lower than 10 mA) that preferentially interplay with the seeds. Since the resistive and capacitive components of the seeds modify the C2AP electrical properties, impedance measurements have been carried out to measure their main dielectric parameters before/after plasma exposure (seeds resistance, capacitance, complex relative permittivity, tangent loss and conductivity). WC significantly changes dielectric losses at low frequencies (< 1 kHz) due to polarization relaxation of the polar molecules (i.e. water). LCRmetry further reveals that C2AP does not substantially alter seeds dielectric parameters, i.e. it neither adds or removes significant amounts of new materials, meaning that the relative starch, protein and lipid contents remain essentially unaffected. However, it cannot be discounted that some bulk properties of the Arabidopsis seeds may be modified, especially regarding their porosity. This characteristic could facilitate penetration of plasma-generated reactive oxygen species into the internal seed tissues, leading to the grafting of oxygenated groups. Water sorption isotherms have been achieved on Arabidopsis seeds and fitted with four thermodynamic models, including the Brunauer-Emmett-Teller model and the Generalized D'Arcy and Watt model. It is demonstrated that C2AP primarily strengthens water-seed affinity by modifying molecular interactions rather than changing the seed's moisture layer.