Flipping Out: Role of Arginine in Hydrophobic Interactions and Biological Formulation Design

TL;DR

This study investigates how arginine influences hydrophobic interactions and protein stability, revealing that it employs both direct and indirect mechanisms that vary with concentration, informing better biological formulation design.

Contribution

The paper uncovers the dual mechanisms of arginine's effects on hydrophobic interactions, demonstrating their coexistence and dependence on concentration, which advances understanding of its role in formulation stability.

Findings

Arginine stabilizes hydrophobic polymer collapse via direct and indirect mechanisms.

High concentrations of arginine destabilize polymer collapse when partial charges are added.

Arginine's effects on virus destabilization and protein stabilization are concentration-dependent.

Abstract

Arginine has been a mainstay in biological formulation development for decades. To date, the way arginine modulates protein stability has been widely studied and debated. Here, we employed a hydrophobic polymer to decouple hydrophobic effects from other interactions relevant to protein folding. While existing hypotheses for the effects of arginine can generally be categorized as either direct or indirect, our results indicate that direct and indirect mechanisms of arginine co-exist and oppose each other. At low concentrations, arginine was observed to stabilize hydrophobic polymer collapse via a sidechain-dominated direct mechanism, while at high concentrations, arginine stabilized polymer collapse via a backbone-dominated indirect mechanism. When adding partial charges to sites on the polymer, arginine destabilized polymer collapse. Further, we found arginine-induced destabilization of a model virus similar to direct-mechanism destabilization of the charged polymer, and concentration-dependent stabilization of a model protein similar to the indirect mechanism of hydrophobic polymer stabilization. These findings highlight the modular nature of the widely used additive arginine, with relevance in the design of stable biological formulations.