CardioGenAI: A Machine Learning-Based Framework for Re-Engineering Drugs for Reduced hERG Liability

TL;DR

CardioGenAI is a machine learning framework that predicts and redesigns drugs to reduce hERG channel activity, thereby decreasing arrhythmia risk while maintaining pharmacological efficacy, demonstrated on an antipsychotic drug.

Contribution

The paper introduces a novel machine learning framework for re-engineering drugs to lower hERG liability without losing therapeutic activity, including a case study on pimozide.

Findings

Generated 100 candidate compounds with reduced hERG activity.

Identified fluspirilene as a promising candidate with 700-fold weaker hERG binding.

Open-sourced the software for broader use in drug discovery.

Abstract

The link between in vitro hERG ion channel inhibition and subsequent in vivo QT interval prolongation, a critical risk factor for the development of arrythmias such as Torsade de Pointes, is so well established that in vitro hERG activity alone is often sufficient to end the development of an otherwise promising drug candidate. It is therefore of tremendous interest to develop advanced methods for identifying hERG-active compounds in the early stages of drug development, as well as for proposing redesigned compounds with reduced hERG liability and preserved on-target potency. In this work, we present CardioGenAI, a machine learning-based framework for re-engineering both developmental and commercially available drugs for reduced hERG activity while preserving their pharmacological activity. The framework incorporates novel state-of-the-art discriminative models for predicting hERG channel activity, as well as activity against the voltage-gated NaV1.5 and CaV1.2 channels due to their potential implications in modulating the arrhythmogenic potential induced by hERG channel blockade. We applied the complete framework to pimozide, an FDA-approved antipsychotic agent that demonstrates high affinity to the hERG channel, and generated 100 refined candidates. Remarkably, among the candidates is fluspirilene, a compound which is of the same class of drugs (diphenylmethanes) as pimozide and therefore has similar pharmacological activity, yet exhibits over 700-fold weaker binding to hERG. We envision that this method can effectively be applied to developmental compounds exhibiting hERG liabilities to provide a means of rescuing drug development programs that have stalled due to hERG-related safety concerns. We have made all of our software open-source to facilitate integration of the CardioGenAI framework for molecular hypothesis generation into drug discovery workflows.