Methylation Operation Wizard (MeOW): Identification of differentially methylated regions in long-read sequencing data

TL;DR

MeOW is a new tool that leverages long-read sequencing data to accurately identify and prioritize differentially methylated regions across the genome, enhancing epigenetic analysis capabilities.

Contribution

It introduces a novel software that utilizes long-read sequencing data for the detection of DMRs, accommodating different input formats for flexibility.

Findings

Successfully identifies DMRs genome-wide

Supports multiple input formats including count files and bam files

Enhances methylation analysis with long-read sequencing data

Abstract

Long-read sequencing (LRS) is able to simultaneously capture information about both DNA sequence and modifications, such as CpG methylation in a single sequencing experiment. Here we present Methylation Operation Wizard (MeOW), a program to identify and prioritize differentially methylated regions (DMRs) genome-wide using LRS data. MeOW can be run using either a file containing counts of per-nucleotide methylated CpG sites or with a bam file containing modified base tags.