Microsecond Time-Resolved Cryo-Electron Microscopy

TL;DR

Microsecond time-resolved cryo-electron microscopy is a novel technique that enables direct observation of protein dynamics with high temporal and spatial resolution, advancing understanding of protein functions.

Contribution

This review summarizes the properties, implementations, and strategies of microsecond time-resolved cryo-EM, highlighting its potential through a case study on CCMV capsid dynamics.

Findings

Demonstrates microsecond temporal resolution in cryo-EM

Provides insights into protein capsid dynamics

Shows potential for studying fast biological processes

Abstract

Microsecond time-resolved cryo-electron microscopy has emerged as a novel approach for directly observing proteins dynamics. By providing microsecond temporal and near-atomic spatial resolution, it has the potential to elucidate a wide range of dynamics that were previously inaccessible and therefore, to significantly advance our understanding of protein function. This review summarizes the properties of the laser melting and revitrification process that underlies the technique and describes different experimental implementations. Strategies for initiating and probing dynamics are discussed. Finally, the microsecond time-resolved observation of the capsid dynamics of CCMV, an icosahedral plant virus, is reviewed, which illustrates important features of the technique as well as its potential.