Analysis of Compositional Data with Positive Correlations among Components using a Nested Dirichlet Distribution with Application to a Morris Water Maze Experiment

TL;DR

This paper introduces a new statistical test for compositional data, specifically addressing positive correlations among components, and applies it to analyze Morris water maze experiment data, revealing different conclusions from prior methods.

Contribution

The paper proposes a novel two-sample test for compositional data based on nested Dirichlet distributions, improving analysis of dependent components in experiments.

Findings

The new test detects differences in group proportions more accurately.

Reanalysis of previous data yields different conclusions.

Highlights flaws in existing Dirichlet-based hypothesis testing methods.

Abstract

In a typical Morris water maze experiment, a mouse is placed in a circular water tank and allowed to swim freely until it finds a platform, triggering a route of escape from the tank. For reference purposes, the tank is divided into four quadrants: the target quadrant where the trigger to escape resides, the opposite quadrant to the target, and two adjacent quadrants. Several response variables can be measured: the amount of time that a mouse spends in different quadrants of the water tank, the number of times the mouse crosses from one quadrant to another, or how quickly a mouse triggers an escape from the tank. When considering time within each quadrant, it is hypothesized that normal mice will spend smaller amounts of time in quadrants that do not contain the escape route, while mice with an acquired or induced mental deficiency will spend equal time in all quadrants of the tank. Clearly, proportion of time in the quadrants must sum to one and are therefore statistically dependent; however, most analyses of data from this experiment treat time in quadrants as statistically independent. A recent paper introduced a hypothesis testing method that involves fitting such data to a Dirichlet distribution. While an improvement over studies that ignore the compositional structure of the data, we show that methodology is flawed. We introduce a two-sample test to detect differences in proportion of components for two independent groups where both groups are from either a Dirichlet or nested Dirichlet distribution. This new test is used to reanalyze the data from a previous study and come to a different conclusion.