LIPSS pattern induced by polymer surface instability for myoblast cell guidance

TL;DR

This study demonstrates how laser-induced periodic surface structures (LIPSS) on polymers can guide myoblast cell growth and improve surface properties, with potential applications in tissue engineering.

Contribution

It introduces a method to create diverse LIPSS patterns on polymers and evaluates their effects on cell guidance and surface chemistry.

Findings

LIPSS patterns influence myoblast alignment and growth.

Laser treatment increases surface oxygen and hydrophilicity.

Different patterns have distinct effects on cell compatibility.

Abstract

The presented study highlights the efficiency of employing a KrF excimer laser to create diverse types of periodic nanostructures (LIPSS - laser induced periodic surface structures) on polyether ether ketone (PEEK) and polyethylene naphthalate (PEN) substrates. By exposing the polymer films below their ablation threshold to laser fluence ranging from 4 to 16 mJcm-2 at 6,000 pulses, we studied both single-phase exposure at beam incidence angles of 0deg and 45deg, and two-phase exposure. Atomic force microscopy analysis revealed that the laser-treated samples contained distinctive periodic patterns such as waves, globules, and pod-like structures each exhibiting unique surface roughness. Moreover, using analytical methods like EDS and XPS shed light on the changes in the atomic composition, specifically focusing on the C and O elements, as a result of laser exposure. Notably, in almost all cases, we observed an increase in oxygen percentage on the sample surfaces. This increase not only led to a decrease in the contact angle with water but also lowered the zeta potential value, thus showing that the modified samples have enhanced hydrophilicity of the surface and altered electrostatic properties. Last but not least, the samples were assessed for biocompatibility; we studied the interaction of the prepared replicates with mouse myoblasts (C2C12). The impact of globular/dot structures on the cell growth in comparison to pristine or linear LIPSS-patterned surfaces was determined. The linear pattern (LIPSS) induced the myoblast cell alignment along the pattern direction, while dot/globular pattern even enhanced the cytocompatibility compared to LIPSS samples.