Identification of urinary biomarkers of food intake for onion by untargeted LC-MS metabolomics

TL;DR

This study identified urinary biomarkers for recent onion intake using untargeted metabolomics in a controlled trial, highlighting specific cysteine derivatives as promising indicators with high predictive accuracy.

Contribution

The paper introduces novel urinary biomarkers for onion intake, discovered through untargeted LC-MS metabolomics in a controlled human trial, demonstrating their specificity and potential for dietary assessment.

Findings

Six biomarkers from cysteine derivatives identified

Biomarkers excreted within 24 hours, indicating recent intake

High predictive accuracy with AUC up to 1

Abstract

Scope: Biomarkers of food intake (BFIs) are useful tools for objective assessment of food intake and compliance. The aim of this study was to discover and identify urinary BFIs for onion. Methods and results: In a randomized controlled cross-over trial, 6 overweight participants (age 24-62 years) consumed meals with 20 g/d onion powder or no onion for 2 weeks. Untargeted UPLC-qTOF-MS metabolic profiling analysis was performed on urine samples and the profiles were analysed by multilevel-PLSDA, modified PLS, and nearest shrunken centroid to select features associated with onion intake. Eight biomarkers were tentatively identified; six of them originated from S-substituted cysteine derivatives such as isoalliin and propiin, which are considered the most specific for onion intake. Most of the biomarkers were completely excreted within 24 hours and no accumulation was observed during 2 weeks indicating their ability to reflect only recent intake of onions. Receiver-operator curves were made to evaluate the performance of individual biomarkers for predicting onion intake. The area under the curve values for these biomarkers ranged from 0.81 to 1. Conclusion: Promising biomarkers of recent onion intake have been identified in human urine. Further studies with complex diets are needed to validate the robustness of these biomarkers.