Estimation of In-Vitro Free Radical Scavenging and Cytotoxic Activities of Thiocarbohydrazones Derivatives

TL;DR

This study evaluates eighteen thiocarbohydrazones derivatives for their antioxidant and cytotoxic activities, finding some compounds with significant free radical scavenging and non-cytotoxic properties suitable for further in vivo testing.

Contribution

It is the first comprehensive in vitro assessment of thiocarbohydrazones derivatives for antioxidant potential and cytotoxicity, highlighting promising candidates for oxidative stress therapy.

Findings

Several compounds showed significant radical scavenging activity.

Selected derivatives were non-cytotoxic to fibroblast cells.

Potential for further in vivo evaluation as therapeutic agents.

Abstract

Objective: To investigate free radical scavenging potential of thiocarbohydrazones derivatives for the discovery of antioxidant compounds of synthetic origin. Method: Eighteen thiocarbohydrazones derivatives were screened for antioxidant activities by using in vitro radical inhibition assay. Cytotoxicity all derivatives were evaluated using invitro cytotoxicity assay on mouse fibroblast cell line. Results: various compounds of the have shown significant radical scavenging activities as compared with the standard drug butylated hydroxytoluene. In addition in an in vitro cell cytotoxicity assay selected analogues were found to be non-cytotoxic against fibroblast 3T3 cell line. Conclusion: Taken together, these findings all compound this series could be further evaluated in in vivo model of oxidative stress to develop as a therapeutic agent against oxidative stress related disorders due to exhibiting excellent free radical scavenging properties and having their non cytotoxic nature against normal cells.