Combining optical diffraction tomography with imaging flow cytometry for characterizing morphology, hemoglobin content, and membrane deformability of live red blood cells

TL;DR

This paper presents a label-free method combining optical diffraction tomography and flow cytometry to accurately reconstruct 3D morphology and hemoglobin content of live red blood cells, enabling detection of membrane deformability changes.

Contribution

It introduces an improved technique for estimating RBC rotation angles and demonstrates 3D reconstruction of healthy and treated RBCs for assessing morphological and biochemical changes.

Findings

Accurate 3D reconstructions of RBCs achieved

Quantification of morphological and hemoglobin content changes

Detection of membrane fluctuation alterations due to treatments

Abstract

Integrating optical diffraction tomography with imaging flow cytometry enables label-free quantifications of the three-dimensional (3D) morphology and hemoglobin content of red blood cells (RBCs) in their natural form. Self-rotation of RBCs flowing in a microfluidic channel has been utilized to achieve various projection directions for 3D reconstruction. However, the practicality of this technique has not been sufficiently studied. We improved the accuracy of estimating the rotation angle of RBCs and demonstrated 3D reconstructions of both healthy and glutaraldehyde-treated RBCs. Results showed the capability to quantify changes in RBC morphology, hemoglobin content, and membrane fluctuations generated by glutaraldehyde treatments, demonstrating the potential to detect changes frequently present in various RBC membrane disorders.