Imaging structurally dynamic ribosomes with cryogenic electron microscopy

TL;DR

This paper reviews how cryogenic electron microscopy has advanced understanding of ribosome structure, dynamics, and biogenesis, especially in prokaryotes, and discusses future prospects for cellular context imaging.

Contribution

It provides a historical and technical overview of cryo-EM applications in ribosome research, emphasizing recent innovations and future directions.

Findings

Insights into ribosome assembly and function

Role of transient biogenesis factors elucidated

Structural variations between strains and species identified

Abstract

Throughout the history of electron microscopy, ribosomes have served as an ideal subject for imaging and technological development, which in turn has driven our understanding of ribosomal biology. Here, we provide a historical perspective at the intersection of electron microscopy technology development and ribosome biology and reflect on how this technique has shed light on each stage of the life cycle of this dynamic macromolecular machine. With an emphasis on prokaryotic systems, we specifically describe how pairing cryo-EM with clever experimental design, time-resolved techniques, and next-generation heterogeneous structural analysis has afforded insights into the modular nature of assembly, the roles of the many transient biogenesis and translation co-factors, and the subtle variations in structure and function between strains and species. The work concludes with a prospective outlook on the field, highlighting the pivotal role cryogenic electron tomography is playing in adding cellular context to our understanding of ribosomal life cycles, and noting how this exciting technology promises to bridge the gap between cellular and structural biology.