Enhancing Cell Proliferation and Migration by MIR-Carbonyl Vibrational Coupling: Insights from Transcriptome Profiling

TL;DR

This study demonstrates that MIR vibrational coupling at 5.6 micrometers significantly enhances fibroblast proliferation and migration, revealing molecular mechanisms and potential for non-thermal therapeutic applications.

Contribution

It provides the first detailed transcriptome analysis of MIR vibrational coupling effects on cells, elucidating molecular pathways involved in cell regulation.

Findings

Fibroblast proliferation increased by 156%.

Migration capacity increased by 162.5%.

Transcriptome analysis reveals molecular mechanisms.

Abstract

Cell proliferation and migration highly relate to normal tissue self-healing, therefore it is highly significant for artificial controlling. Recently, vibrational strong coupling between biomolecules and Mid-infrared (MIR) light photons has been successfully used to modify in vitro bioreactions, neuronal signaling and even animal behavior. However, the synergistic effects from molecules to cells remains unclear, and the regulation of MIR on cells needs to be explained from the molecular level. Herein, the proliferation rate and migration capacity of fibroblasts were increased by 156% and 162.5%, respectively, by vibratory coupling of 5.6 micrometers photons with carbonyl groups in biomolecules. Through transcriptome sequencing analysis, the regulatory mechanism of infrared light in 5.6 micrometers was explained from the level of signal pathway and cell components. 5.6 micrometers optical high power lasers can regulate cell function through vibrational strong coupling while minimizing photothermal damage. This work not only sheds light on the non-thermal effect on MIR light-based on wound healing, but also provides new evidence to future frequency medicine.