Optical Diffraction Tomography Meets Fluorescence Localization Microscopy

TL;DR

This paper introduces a novel method that combines optical diffraction tomography with fluorescence localization microscopy to extract structural information from single molecule data, enabling joint estimation of refractive index and molecule positions.

Contribution

It presents a new joint optimization framework that interprets SMLM data as measures in an optical diffraction tomography system, allowing simultaneous reconstruction of structure and molecule locations.

Findings

Successful joint estimation of refractive index and molecule positions.

Enhanced structural information extraction from SMLM data.

Framework applicable to single frame SMLM data.

Abstract

We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the illumination sources are fluorophores within the sample. Building upon this model, we propose a joint optimization framework to estimate both the refractive index map and the position of fluorescent molecules from the sole SMLM frames.