Brillouin Gain Microscopy

TL;DR

Brillouin gain microscopy (BGM) offers high temporal and spatial resolution for contactless mechanical imaging of materials and biological samples by detecting Brillouin gain at specific frequencies.

Contribution

This paper introduces a novel BGM technique with 200-fold higher temporal resolution for detailed mechanical imaging.

Findings

Successful imaging of materials, organisms, and cells with high spectro-spatiotemporal resolution

Enhanced sensitivity in detecting Brillouin gain at specific frequencies

Potential for advanced material and biological research applications

Abstract

Optical imaging with mechanical contrast is critical for material and biological discovery since it allows contactless light-radiation force-excitation within the sample, as opposed to traditional mechanical imaging. Whilst optical microscopy based on stimulated Brillouin scattering (SBS) enables mechanical imaging of materials and living biological systems with high spectrospatial resolution, its temporal resolution remains limited. Here, we develop Brillouin gain microscopy (BGM) with a 200-fold higher temporal resolution by detecting the Brillouin gain at a mechanically contrasting frequency in the sample with high sensitivity. Using BGM, we demonstrate mechanical imaging of materials, living organisms and cells at high spectro-spatiotemporal resolution.