Biological rhythms generated by a single activator-repressor loop with heterogeneity and diffusion

TL;DR

This paper demonstrates that a single activator-repressor loop, with spatial separation and diffusion, can generate sustained biological oscillations, simplifying the understanding of circadian rhythms and minimal biological clocks.

Contribution

It introduces a minimal model showing that a single reaction pair with spatial separation and diffusion can produce oscillations, reducing complexity in biological clock models.

Findings

A single activation-repression pair can sustain oscillations.

Spatial separation and diffusion are sufficient for rhythmic behavior.

Simplifies the conceptual basis for biological oscillators.

Abstract

Common models of circadian rhythms are constructed as compartmental reactions of well mixed biochemicals involving a negative-feedback loop containing several intermediate reaction steps in order to enable oscillations. Spatial transport of reactants is mimicked as an extra compartmental reaction step. In this letter, we show that a single activation-repression biochemical reaction pair is enough to produce sustained oscillations, if the sites of both reactions are spatially separated and molecular transport is mediated by diffusion. Our proposed scenario is the simplest possible one in terms of the participating chemical reactions and provides a conceptual basis for understanding biological oscillations and triggering in-vitro assays aimed at constructing minimal clocks.