Effective viscosity of methyl cellulose solutions in phosphate buffered saline in real-time deformability cytometry

TL;DR

This paper derives equations to determine the effective viscosity of methyl cellulose solutions in PBS during real-time deformability cytometry, enabling better measurement of cell mechanical properties.

Contribution

It introduces a new method to calculate effective viscosity in RT-DC experiments, improving accuracy over existing models.

Findings

Derived equations for effective viscosity in MC-PBS solutions

Compared new viscosity model with existing models

Facilitates accurate Young's modulus measurements in RT-DC

Abstract

Here, we derive the equations to calculate the effective viscosity of solutions of methyl cellulose (MC) dissolved in phosphate buffered saline (PBS) in real-time deformability cytometry (RT-DC) experiments. The calculations are based on the rheometer measurements described by B\"uy\"ukurganci et al. (2022). We outline how to get the final equations and compare the results to the current viscosity model from Herold (2017). These viscosity functions will be used to determine the Young's moduli of biological cells and other soft materials from RT-DC experiments.