A low-cost confocal microscope for the undergraduate lab
A. Reguilon, W. Bethard, and E. Brekke

TL;DR
This paper presents a low-cost, easy-to-assemble confocal microscope designed for undergraduate physics labs, enabling students to explore advanced microscopy concepts with affordable, commercially available parts and 3D-printed components.
Contribution
It introduces a simple, cost-effective confocal microscope setup that enhances physics education through hands-on experiments and accessible design.
Findings
Successful measurement of slide thickness and surface topography
Demonstration of depth perception and data acquisition capabilities
Cost reduction through use of 3D-printed parts
Abstract
We demonstrate a simple and cost-efficient scanning confocal microscope setup for use in advanced instructional physics laboratories. The setup is constructed from readily available commercial products, and the implementation of a 3D-printed flexure stage allows for further cost reduction and pedagogical opportunity. Experiments exploring the thickness of a microscope slide and the surface of solid objects with height variation are presented as foundational components of undergraduate laboratory projects, and demonstrate the capabilities of a confocal microscope. This system allows observation of key components of a confocal microscope, including depth perception and data acquisition via transverse scanning, making it an excellent pedagogical resource.
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Taxonomy
TopicsImage Processing Techniques and Applications · Advanced Fluorescence Microscopy Techniques · Optical Coherence Tomography Applications
