Continuous odor profile monitoring to study olfactory navigation in small animals

TL;DR

This paper introduces a novel method for continuous, precise measurement and control of airborne odor concentrations in behavioral arenas, enabling detailed study of olfactory navigation in small model animals like C. elegans and D. melanogaster larvae.

Contribution

The study presents a new approach combining spatially patterned odor delivery with distributed gas sensors for real-time odor profile monitoring during animal behavior experiments.

Findings

Accurate, continuous odor measurement across the arena.

Insights into chemotaxis strategies of C. elegans and D. melanogaster larvae.

Enhanced control of odor landscapes for behavioral studies.

Abstract

Olfactory navigation is observed across species and plays a crucial role in locating resources for survival. In the laboratory, understanding the behavioral strategies and neural circuits underlying odor-taxis requires a detailed understanding of the animal's sensory environment. For small model organisms like C. elegans and larval D. melanogaster, controlling and measuring the odor environment experienced by the animal can be challenging, especially for airborne odors, which are subject to subtle effects from airflow, temperature variation, and from the odor's adhesion, adsorption or reemission. Here we present a method to flexibly control and precisely measure airborne odor concentration in an arena with agar while imaging animal behavior. Crucially and unlike previous methods, our method allows continuous monitoring of the odor profile during behavior. We construct stationary chemical landscapes in an odor flow chamber through spatially patterned odorized air. The odor concentration is measured with a spatially distributed array of digital gas sensors. Careful placement of the sensors allows the odor concentration across the arena to be accurately inferred and continuously monitored at all points in time. We use this approach to measure the precise odor concentration that each animal experiences as it undergoes chemotaxis behavior and report chemotaxis strategies for C. elegans and D. melanogaster larvae populations under different spatial odor landscapes.