K-space interpretation of image-scanning-microscopy

TL;DR

This paper presents a k-space analysis of image-scanning microscopy (ISM), revealing its equivalence to synthetic-aperture radar and oblique-illumination microscopy, and demonstrates that ISM can be performed with a single detector in k-space.

Contribution

The paper introduces a novel k-space perspective of ISM, showing it can be implemented with a single detector in k-space, enhancing understanding and potential simplifications.

Findings

ISM is equivalent to spotlight SAR and oblique-illumination microscopy.

ISM can be performed with a single detector in k-space, as demonstrated numerically.

The k-space analysis provides new insights into ISM's capabilities.

Abstract

In recent years, image-scanning microscopy (ISM, also termed pixel-reassignment microscopy) has emerged as a technique that improves the resolution and signal-to-noise compared to confocal and widefield microscopy by employing a detector array at the image plane of a confocal laser scanning microscope. Here, we present a k-space analysis of coherent ISM, showing that ISM is equivalent to spotlight synthetic-aperture radar (SAR) and analogous to oblique-illumination microscopy. This insight indicates that ISM can be performed with a single detector placed in the k-space of the sample, which we numerically demonstrate.