Development of a Novel Platform for in vitro Electrophysiological Recording

TL;DR

This thesis presents a new platform using meta-electrodes and laser optoporation for high-quality intracellular recordings from hiPSCs-CMs, enhancing electrophysiological studies with minimal invasiveness.

Contribution

Introduces a novel method combining meta-electrodes and laser optoporation for intracellular recording on commercial CMOS-MEAs without device rework.

Findings

Successful high-quality intracellular recordings from hiPSCs-CMs.

Compatibility of the method with existing commercial MEA platforms.

Potential for improved cardiac electrophysiological research.

Abstract

This PhD thesis introduces the concept of meta-electrodes coupled with laser optoporation for high quality intracellular signals from hiPSCs-CM. These signals can be recorded on high-density commercial CMOS-MEAs from 3Brain characterized by thousands of electrode covered by a thin film of porous Platinum without any rework of the devices, 3D nanostructures or circuitry for electroporation. Subsequently, I attempted to translate these unique features of low invasiveness and reliability to other commercial MEA platforms, in order to develop a new tool for cardiac electrophysiological accurate recordings.