Fluorescent property of carbon dots extracted from cigarette smoke and the application in bio-imaging

TL;DR

This study extracts fluorescent carbon dots from cigarette smoke, enhances their properties through reduction, and demonstrates their potential for bio-imaging applications with low toxicity and high fluorescence.

Contribution

It is the first to extract and modify cigarette smoke-derived carbon dots for effective bio-imaging, highlighting their potential in cancer diagnosis and treatment.

Findings

Cigarette smoke contains nanoscale carbon dots with strong fluorescence.

Reduction with sodium borohydride significantly increases fluorescence intensity.

R-CDs can penetrate cells and visualize cell structures clearly.

Abstract

Cigarette smoke is one of the six major pollution sources in the room air. It contains large number of particles with size less than 10 nm. There exist carbon dots (CDs) in cigarette smoke which have strong fluorescence and with good bio-compatibility and low toxicity. CDs in cigarette smoke can be applied in bio-imaging which has great potential applications in the integration of cancer diagnosis and treatment. In this paper, CDs were extracted from cigarette smoke. Then, sodium borohydride was added to CDs aqueous solution for reduction and the reduced CDs (R-CDs) were used for biological cell imaging. The results indicate that the CDs with the particle size $<$10 nm in cigarette smoke are self-assembled by the polymerizated polycyclic aromatic hydrocarbons (PAHs) and ammonium nitrite which are disk nano-structure composed of $sp^2$/$sp^3$ carbon and oxygen/nitrogen groups or polymers. Sodium borohydride can reduce the carbonyl group on the surface of CDs to hydroxyl group and increase the ratio of the Na 1s ratio of the CDs from 1.86 to 7.42. The CDs can emit blue fluorescence under ultraviolet irradiation. After reduction, the R-CDS have the intensity of fluorescence 7.2 times than before and the fluorescence quantum yield increase from 6.13\% to 8.86\%. The photoluminescence (PL) wavelength of R-CDS have red-shift of 7 nm which was due to the increasing of Na element ratio. The onion epidermal cells labeled with R-CDs show that the CDs could pass through the cell wall into the cell and reach the nucleus. The cell wall and the nucleus could be clearly visualized. CDs also shows low toxicity to human bronchial epithelial cells (BEAS-2B) with good biological activity. The obtained results indicate that the CDs and R-CDs have good fluorescent property which could be used as bio-imaging agent.