Asymmetric split-ring plasmonic nanostructures for optical sensing of Escherichia coli

TL;DR

This paper presents a novel Fano-resonant metamaterial platform that enhances Raman signals for detecting Escherichia coli in liquid, enabling ultrasensitive, label-free bacterial identification suitable for diagnostics and environmental monitoring.

Contribution

The work introduces a Fano-enhanced Raman scattering platform using asymmetric split-ring plasmonic nanostructures for improved bacterial detection.

Findings

Raman signatures of E. coli were successfully recorded using the platform.

FERS signal increased during bacterial exponential growth phase.

The platform operates effectively at off-resonance excitation, reducing photodamage.

Abstract

Strategies for in-liquid micro-organism detection are crucial for the clinical and pharmaceutical industries. While Raman spectroscopy is a promising label-free technique for micro-organism detection, it remains challenging due to the weak bacterial Raman signals. In this work, we exploit the unique electromagnetic properties of metamaterials to identify bacterial components in liquid using an array of Fano-resonant metamolecules. This Fano-enhanced Raman scattering (FERS) platform is designed to exhibit a Fano resonance close to the protein amide group fingerprint around 6030 nm. Raman signatures of Escherichia coli were recorded at several locations on the metamaterial under off-resonance laser excitation at 530 nm, where the photodamage effect is minimized. As the sizes of the Escherichia coli are comparable to the micro-gaps, i.e 0.41 {\mu}m, of the metamaterials, its local immobilisation leads to an increase in the Raman sensitivity. We also observed that the time-dependent FERS signal related to bacterial amide peaks increased during the bacteria's mid-exponential phase while it decreased during the stationary phase. This work provides a new set of opportunities for developing ultrasensitive FERS platforms suitable for large-scale applications and could be particularly useful for diagnostics and environmental studies at off-resonance excitation.