Surface plasmon resonance biosensors based on Kretschmann configuration: basic instrumentation and applications

TL;DR

This paper reviews the Kretschmann configuration-based Surface Plasmon Resonance biosensors, highlighting their instrumentation, recent advancements, and diverse applications in label-free biomolecular interaction analysis.

Contribution

It provides a comprehensive overview of the basic instrumentation and recent developments in Kretschmann configuration-based SPR biosensors.

Findings

Kretschmann configuration offers high sensitivity and real-time detection.

Various applications in biomolecular interaction studies.

Recent trends improve sensor performance and usability.

Abstract

Surface Plasmon Resonance (SPR) offers a powerful tool for label-free and non-invasive characterization of biomolecular interactions. To date, several experimental configurations, based on two fundamental physical phenomena, e.g., attenuated total reflection and diffraction, have been developed to measure the SPR signal generated due to the resonant interactions between incident light and plasma waves on the metal surface. These configurations are divided into three categories: grating-based, prism-based, and waveguide-based coupling. Among such techniques, one of the prism-based SPR coupling schemes, popularly known as Kretschmann configuration, is most widely used due to its high sensitivity, operational simplicity, lower cost, and real-time detection. This chapter explains the basic instrumentation and reviews the recent trends in the development of Kretschmann configuration-based SPR biosensors with its applications.