The development of food protein-inorganic hybrid nanoflowers with outstanding role in stabilizing natural pigments

TL;DR

This study develops food protein-inorganic hybrid nanoflowers (HNFs) using enzymatic hydrolysis, enhancing their ability to stabilize and deliver natural pigments like Monascus red and curcumin in food and cosmetic applications.

Contribution

It introduces a method to produce versatile HNFs from various food proteins via enzymatic hydrolysis, expanding their application in pigment stabilization and delivery.

Findings

HNF with soy protein hydrolysate shows high loading capacity for Monascus red.

HNF effectively stabilizes lipophilic curcumin in harsh environments.

Enzymatic hydrolysis broadens protein sources for HNF fabrication.

Abstract

Protein-inorganic hybrid nanoflowers (HNFs) possess unique properties in promoting surface reaction and have attracted wide-spread attention as a newly developed nanomaterial. However, the availability of protein sources has up to now been mostly limited to enzymes, which narrows the application of HNFs especially in food industry. Here we show that for many types of food protein, enzymatic hydrolysis can improve its ability to form versatile HNFs, or even induce HNF formation where the protein source did not show its formation a priori. The treatment of enzymatic hydrolysis increases the flexibility of such proteins and induces nucleation sites of HNFs in the early formation stage by decomposing those proteins into polypeptides. In particular, the HNF prepared with soy protein hydrolysate further shows a high loading capacity of water-soluble Monascus red, reaching up to 554.1 mg per gram of HNF. Its stabilization towards lipophilic curcumin is similarly impressive with the loading capacity reaching 21.9 mg per gram of HNF. This HNF could also effectively protect these two sensitive natural pigments in harsh environments. This research significantly broadens the available protein source for HNF fabrication and demonstrates the potential of HNFs as novel protein-based delivery systems - especially for sensitive natural pigments - which could serve the fields of food, cosmetic and medicine.