5-Minute Magnetic Particle Spectroscopy (MPS)-based Bioassay: Towards Ultra-fast, Convenient, and Large-scale Disease Diagnosis

TL;DR

This paper introduces a rapid 5-minute magnetic particle spectroscopy-based bioassay for quick and large-scale disease detection, demonstrating its effectiveness in detecting SARS-CoV-2 spike protein with high sensitivity.

Contribution

The study presents a novel ultra-fast bioassay method using magnetic particle spectroscopy that significantly reduces detection time for liquid biosensors.

Findings

Detection of SARS-CoV-2 spike protein below 5 nM

Assay time reduced to 5 minutes

Potential application to various biosensors

Abstract

In this work, we report a 5-minute magnetic particle spectroscopy (MPS)-based bioassay strategy. In our approach, surface functionalized magnetic nanoparticles (MNPs) are incubated with target analytes at 37 {\deg}C with agitation for 3 minutes and the MPS reading is then taken at the 5th minute. We have proved the feasibility of 5-minute ultra-fast detection of SARS-CoV-2 spike protein with a detection limit below 5 nM (0.2 pmole). Our proposed 5-minute bioassay strategy may be applied to reduce assay time for other liquid phase, volumetric biosensors such as nuclear magnetic resonance (NMR), quantum dots (QD), fluorescent biosensors, etc.