Asymptotic decoupling of population growth rate and cell size distribution

TL;DR

This paper analytically demonstrates that in balanced bacterial growth, population growth rate and cell size distribution decouple, with growth rate depending solely on single-cell dynamics, revealing how fluctuations influence population behavior.

Contribution

It provides the first analytic solutions showing the decoupling of population growth rate and cell size distribution in fluctuating bacterial populations.

Findings

Population growth rate depends only on single-cell growth process.

Cell size distribution depends on division and size regulation models.

Growth rate variability increases the difference between population and single-cell growth rates.

Abstract

The rate at which individual bacterial cells grow depends on the concentrations of cellular components such as ribosomes and proteins. These concentrations continuously fluctuate over time and are inherited from mother to daughter cells, leading to correlations between the growth rates of cells across generations. Division sizes of cells are also stochastic and correlated across generations due to a phenomenon known as cell size regulation. Fluctuations and correlations from both growth and division processes affect the population dynamics of an exponentially growing culture. Here, we provide analytic solutions for the population dynamics of cells with continuously fluctuating growth rates coupled with a generic model of cell-size regulation. We show that in balanced growth, the effects of growth and division processes decouple; the population growth rate only depends on the single-cell growth rate process, and the population cell size distribution only depends on the model of division and cell size regulation. The population growth rate is always higher than the average single-cell growth rate, and the difference increases with growth rate variability and its correlation time. This difference also sets the timescale for the population to reach its steady state. We provide analytical solutions for oscillations in population growth rate and traveling waves in size distribution during this approach to the steady state.