Skipping the boundary layer: high-speed droplet-based immunoassay using Rayleigh acoustic streaming

TL;DR

This paper introduces a novel droplet-based immunoassay technique that employs Rayleigh acoustic streaming at lower ultrasonic frequencies, significantly increasing assay speed by eliminating boundary layer limitations.

Contribution

The study demonstrates that using Rayleigh streaming instead of traditional Eckart streaming accelerates biosensor assays, achieving a 30-fold speed increase in SARS-CoV-2 antibody detection.

Findings

Rayleigh streaming provides slip velocity, reducing boundary layer effects.

Threefold speedup in droplet mixing compared to Eckart streaming.

Immunoassay time reduced from 20 minutes to 40 seconds.

Abstract

Acoustic mixing of droplets is a promising way to implement biosensors that combine high speed and minimal reagent consumption. To date, this type of droplet mixing is driven by a volume force resulting from the absorption of high-frequency acoustic waves in the bulk of the fluid. Here, we show that the speed of these sensors is limited by the slow advection of analyte to the sensor surface due to the formation of a hydrodynamic boundary layer. We eliminate this hydrodynamic boundary layer by using much lower ultrasonic frequencies to excite the droplet, which drives a Rayleigh streaming that behaves essentially like a slip velocity. Three-dimensional simulations show that this provides a threefold speedup compared to Eckart streaming. Experimentally, we shorten a SARS-CoV-2 antibody immunoassay from 20 min to 40 s.