Multi-modal imaging using a cascaded microscope design

TL;DR

This paper introduces M-FAST, a scalable multimodal imaging system with 96 cameras that captures high-resolution, multi-channel images over large areas, enabling dual-channel fluorescence and phase contrast imaging.

Contribution

The paper presents a novel optical design and system architecture for large-area, high-resolution multimodal imaging using a compact array of cameras and fiber bundles.

Findings

Captures dual-channel fluorescence images at 2.2um resolution

Achieves large 8x10mm^2 field of view

Provides phase contrast measurements

Abstract

We present a new Multimodal Fiber Array Snapshot Technique (M-FAST), based on an array of 96 compact cameras placed behind a primary objective lens and a fiber bundle array. which is capable of large-area, high-resolution, multi-channel video acquisition. The proposed design provides two key improvements to prior cascaded imaging system approaches: a novel optical arrangement that accommodates the use of planar camera arrays, and the new ability to acquire multi-modal image data acquisition. M-FAST is a multi-modal, scalable imaging system that can acquire snapshot dual-channel fluorescence images as well as d phase contrast measurements over a large 8x10mm^2 FOV at 2.2um full-pitch resolution.