Light-regulated adsorption and desorption of Chlamydomonas cells at surfaces

TL;DR

This study investigates how blue and red light regulate the attachment and detachment of Chlamydomonas cells on surfaces, revealing kinetics and mechanisms that could inform biofilm formation understanding.

Contribution

It provides the first population-level analysis of light-controlled surface adhesion in Chlamydomonas, modeling the kinetics with time delays and distinguishing protein-mediated adhesion from phototaxis effects.

Findings

Cell adsorption is faster than desorption.

Light conditions induce a response lag in adhesion dynamics.

Phototaxis does not influence surface adhesion kinetics.

Abstract

Microbial colonization of surfaces represents the first step towards biofilm formation, which is a recurring phenomenon in nature with beneficial and detrimental implications in technological and medical settings. Consequently, there is a current interest in elucidating the fundamental aspects of the initial stages of biofilm formation of microorganisms on solid surfaces. While most of the research is oriented to understand bacterial surface colonization, such observations at a fundamental level using photosynthetic microalgae are thus far elusive. Recent single-cell studies showed that the flagellar adhesion of Chlamydomonas is switched on in blue light and switched off under red light [Kreis et al., Nature Physics, 2018, 14, 45-49]. Here, we study this light-switchable surface association of C. reinhardtii on the population level and measure the kinetics of adsorption and desorption of suspensions of motile cells on glass surfaces using bright field optical microscopy. We observe that both processes exhibit a response lag relative to the time at which the blue- and red-light conditions are set and model this feature using time-delayed Langmuir-type kinetics. We find that cell adsorption occurs significantly faster than desorption, which we attribute to the protein-mediated molecular adhesion mechanism of the cells. Adsorption experiments using phototactically blind Chlamydomonas mutants demonstrate that phototaxis does not affect the cell adsorption kinetics. Hence, this method can be used as an assay for characterizing the dynamics of the surface colonization of microbial species exhibiting light-regulated surface adhesion.