Power Law Rheology of Folded Protein Hydrogels

TL;DR

This study reveals that folded protein hydrogels exhibit consistent power-law viscoelasticity with a dense mesoscopic fractal structure, and their mechanical properties are highly reversible and controllable, highlighting mesoscopic origins of their viscoelastic behavior.

Contribution

The paper demonstrates that folded protein hydrogels have mesoscopic power-law rheology with reversible nonlinear behavior, linking their mechanical properties to their meso-structure.

Findings

Power-law viscoelasticity with exponent β=0.03

Fractal dimension of the structure is 2.48

Reversible stiffening and energy dissipation during deformation

Abstract

Folded protein hydrogels are prime candidates as tuneable biomaterials but it is unclear to what extent their mechanical properties have mesoscopic, as opposed to molecular origins. To address this, we probe hydrogels of the muscle-derived protein $I27_5$, using a multimodal rheology approach. Across multiple protocols, the hydrogels consistently exhibit power-law viscoelasticity in the linear viscoelastic regime with an exponent $\beta = 0.03$, suggesting a dense fractal meso-structure, with predicted fractal dimension $d_f = 2.48$. In the nonlinear viscoelastic regime, the hydrogel undergoes stiffening and energy dissipation, indicating simultaneous alignment and unfolding of the folded proteins. Remarkably, this behaviour is highly reversible, as the value of $\beta$, $d_f$ and the viscoelastic moduli return to their equilibrium value, even after multiple cycles of deformation. This highlights a previously unrevealed diversity of viscoelastic properties that originate on the mesoscopic scale. These considerations are likely to be key to controlling the viscoelasticity of folded protein hydrogels.