Comparing fast imaging techniques for individual pulse imaging by Cherenkov in vivo from electron FLASH irradiation

TL;DR

This study developed a fast, pulse-by-pulse Cherenkov imaging technique using a CMOS camera and intensifier to monitor ultra-high dose rate electron therapy in vivo with submillimeter resolution, capturing respiratory motion and treatment variability.

Contribution

It introduces a novel in vivo Cherenkov imaging method capable of single pulse resolution during FLASH irradiation, enhancing real-time treatment monitoring and potential respiratory gating.

Findings

Intensifier increased signal-to-noise ratio from 15 to 280.

Cherenkov emission profile extended beyond treatment field due to tissue scattering.

Detected respiratory cycle changes of about 3 mm in Cherenkov emission profile.

Abstract

Objective: In this study, a fast imaging technique was developed for the first in vivo Cherenkov emission imaging from an ultra-high dose rate (UHDR) electron beam source at single pulse (360 Hz) submillimeter resolution. Approach: A CMOS camera, gated to the UHDR LINAC, imaged the Cherenkov emission profiles pulse by pulse passively during the irradiation of mice on their limbs and intestinal region. The utility of an intensifier was investigated for its effect on image quality including signal to noise and spatial resolution. Pulse by pulse variability in Cherenkov emission profile were quantified spatially and temporally. Main results: An intensifier improved the emission profile signal to noise ratio from 15 to 280, with reduced spatial resolution. The profile extended beyond of the treatment field due to the lateral scattering of the electrons in tissue and its optical properties. The CMOS camera with an intensifier detected the changes in Cherenkov emission profile during expiration and inspiration of the respiration cycle for the mice to be about 3 mm. Significance: This fast imaging technique can be utilized for in vivo intrafraction monitoring of FLASH patient treatments at single pulse resolution. It can display delivery differences during respiration, and variability in the delivered treatment's surface profile, which may perturb from the intended UHDR treatment more for pencil beam scanning systems. The technique may leverage Cherenkov emission surface profile to gate the treatment delivery via respiratory gating systems under FLASH conditions.