Enhanced precision of circadian rhythm by output system

TL;DR

This study demonstrates that the output system of the circadian clock reduces fluctuations in rhythm period, enhancing accuracy without additional noise reduction, based on numerical and analytical comparisons.

Contribution

It provides a novel analytical and numerical analysis showing the output system improves circadian rhythm precision by reducing stochastic fluctuations.

Findings

Output system decreases fluctuation in circadian period.

Fluctuation reduction occurs at typical degradation rates.

Output system enhances rhythm accuracy without extra denoising.

Abstract

Circadian rhythms are biological rhythms of approximately 24 h that persist even under constant conditions without environmental daily cues. The molecular circadian clock machinery generates the physiological rhythms, which can be transmitted into the downstream output system. Owing to the stochastic nature of the biochemical reactions, the oscillation period of circadian rhythms exhibited by individual organisms or cells is not constant on a daily basis with variations as high as 10 % in terms of the coefficient of variation. Although the fluctuations in circadian rhythm is measured through a reporter such as bioluminescence or fluorescence, experimentally confirming whether the fluctuations found in the reporter system are the same as those in the clock itself is challenging. This study numerically and analytically investigated a coupled system of a circadian clock and its output system, and then compared the fluctuations in the oscillation period of the two systems. We found that the amount of fluctuation in the output system is smaller than that in the circadian clock when the degradation rate of the molecules responsible for the output system was at typical values. The results obtained imply that the output system improves the accuracy of the circadian rhythm without the need for any special denoising processes.