2D mapping of radiation dose and clonogenic survival for accurate assessment of in vitro X-ray GRID irradiation effects

TL;DR

This study develops a framework linking local radiation dose to clonogenic survival in in vitro GRID irradiation, enabling precise assessment of radiobiological effects and showing reduced cell survival at high doses.

Contribution

Introduces a novel image segmentation and dosimetry framework to map surviving colonies and predict survival levels in GRID irradiation experiments.

Findings

Gafchromic film and FLUKA simulations produced similar dose maps.

Linear-quadratic parameters for open field irradiation were determined.

GRID irradiation at high doses reduces cell survival compared to open field.

Abstract

Spatially fractionated radiation therapy (SFRT or GRID) is an approach to deliver high local radiation doses in an 'on-off' pattern. To better appraise the radiobiological effects from GRID, a framework to link local radiation dose to clonogenic survival needs to be developed. A549 (lung) cancer cells were irradiated in T25 cm$^2$ flasks using 220 kV X-rays with an open field or through a tungsten GRID collimator with periodical 5 mm openings and 10 mm blockings. Delivered nominal doses were 2, 5, and 10 Gy. A novel approach for image segmentation was used to locate the centroid of surviving colonies in scanned images of the cell flasks. GafchromicTM film dosimetry (GFD) and FLUKA Monte Carlo (MC) simulations were employed to map the dose distribution in the flasks at each surviving colony centroid. Fitting the linear-quadratic (LQ) function to clonogenic survival data for open field irradiation, the expected survival level at a given dose level was calculated. The expected survival level was then mapped together with the observed levels in the GRID-irradiated flasks. GFD and FLUKA MC gave similar dose distributions, with a mean peak-to-valley dose ratio of about 5. LQ-parameters for open field irradiation gave $\alpha = 0.16 \pm 0.04$ Gy$^{-1}$ and $\beta = 0.001 \pm 0.004$ Gy$^{-2}$. Using the image segmentation method, the mean absolute percentage deviation between observed and predicted survival in the (peak; valley) dose regions was (8; 10) %, (4; 41) %, and (3; 138) % for 2, 5 and 10 Gy, respectively. In conclusion, a framework for mapping of surviving colonies following GRID irradiation together with predicted survival levels from homogeneous irradiation was presented. For the given cell line, our findings indicate that GRID irradiation, especially at high peak doses, causes reduced survival compared to an open field configuration.