A full contraction-reaction-diffusion model for pattern formation in geometrically confined microtissues

TL;DR

This paper introduces a novel reaction-diffusion model that incorporates active contraction and poroelastic properties of cellular tissues to better explain pattern formation in confined microtissues, integrating mechanical and biochemical factors.

Contribution

It develops a coupled contraction-reaction-diffusion model considering tissue activeness and poroelasticity, advancing biophysical realism in pattern formation modeling.

Findings

The model captures pattern formation influenced by mechanical cues.

Mechanical forces regulate chemical transport in tissues.

The approach aligns with experimental observations.

Abstract

The reaction-diffusion models have been extensively applied to explain the mechanism of pattern formations in early embryogenesis based on geometrically confined microtissues consisting of human pluripotent stem cells. Recently, mechanical cues, such as the cellular stresses and strains, have been found to dictate the pattern formation in human stem cell differentiation. As a result, the traditional reaction-diffusion models are modified by adding mechanically related terms to consider the role played by the mechanical cues. However, these models either do not consider the activeness of the cellular tissues or neglect their poroelastic nature that biological tissues are made by both cells and interstitial fluid. Hence, the current models suffer from the lacks of biophysical relevance. Here we propose a modified reaction-diffusion model that couples with the active contraction of cellular tissues. The cellular tissue is modelled as a piece of biphasic poroelastic material, where mechanical forces naturally regulate the transport of chemical cues. Such chemical cues direct cell fate and hence yield certain types of pattern formations observed in previous experiments.