Open Droplet Microfluidics for Testing Multi-Drug Resistance and Antibiotic Resilience in Bacteria

TL;DR

This paper introduces a droplet microfluidics platform that enables rapid, high-throughput testing of bacterial resistance to multiple antibiotics, combining imaging and search algorithms for efficient analysis.

Contribution

It presents a novel multi-drug screening platform integrating droplet microfluidics, imaging, and algorithms to assess bacterial resilience quickly and accurately.

Findings

Tested 12 antibiotic combinations on resistant bacteria

Detected bacterial resistance within 8 hours

Potential application in biofilms and microbial aggregates

Abstract

New combinations of existing antibiotics are being investigated to combat bacterial resilience. This requires detection technologies with reasonable cost, accuracy, resolution, and throughput. Here, we present a multi -drug screening platform for bacterial cultures by combining droplet microfluidics, search algorithms, and imaging with a wide field of view. We remotely alter the chemical microenvironment around cells and test 12 combinations of resistant cell types and chemicals. Fluorescence intensity readouts allow us to infer bacterial resistance to specific antibiotics within 8 hours. The platform has potential to detect and identify parameters of bacterial resilience in cell cultures, biofilms, and microbial aggregates.