Control of magnetic susceptibility of probiotic strain Lactobacillus Rhamnosus GG

TL;DR

This study enhances the magnetic susceptibility of probiotic Lactobacillus rhamnosus GG by modifying growth conditions, enabling potential use in magnetically targeted drug delivery for cancer therapy.

Contribution

It demonstrates that cultivating LGG in a magnetic field and iron-enriched medium significantly increases its magnetic susceptibility, a novel approach for biomedical applications.

Findings

Magnetic susceptibility increased 1.8 times with medium modification.

Susceptibility increased 2.6 times with magnetic field cultivation.

Maximum susceptibility was 4.8 times higher in combined conditions.

Abstract

The paper investigates the increase in the natural magnetically controlled properties of probiotic microorganisms Lactobacillus rhamnosus GG (LGG) and their ability to form magnetic sensitive inclusions (MsI). The magnetic susceptibility of LGG was increased by modifying the nutrient medium composition and by cultivating the probiotic culture in a constant magnetic field. Therefore, this study can be useful for their further use as magnetically controlled vectors, since it is being extensively researched for the use in targeted drug delivery in cancer treatment, for the prevention of chemotherapy complications, etc. The results of this study indicate that growing microorganisms with natural magnetically-guided properties in a modified medium with iron chelate and in an external magnetic field leads to an increase in the magnetic susceptibility of LGG by 1,8 and 2,6 times, respectively, compared with the control. The best magnetic susceptibility was recorded for LGG suspensions, which were grown in a constant magnetic field and cultured in modified medium with iron chelate. The LGG suspension, grown both in a constant magnetic field and on a modified medium with iron chelate, had the highest magnetic susceptibility and was 4.8 times larger than the control.