Chloroplasts in plant cells show active glassy behavior under low light conditions

TL;DR

This study reveals that chloroplasts in plant cells exhibit active glassy behavior under low light, with dynamics resembling colloidal systems near the glass transition, suggesting a physiological role for such phase transitions.

Contribution

The paper uncovers the active glassy dynamics of chloroplasts under dim light and provides a minimal model explaining the parameters controlling their dense arrangement.

Findings

Chloroplasts form a quasi-2D dense layer under low light conditions.

Chloroplast motion exhibits burst-like re-arrangements similar to colloidal glasses.

A minimal model explains the stability and fluidization of chloroplast arrangements.

Abstract

Plants have developed intricate mechanisms to adapt to changing light conditions. Besides photo- and helio- tropism -- the differential growth towards light and the diurnal motion with respect to sunlight -- chloroplast motion acts as a fast mechanism to change the intracellular structure of leaf cells. While chloroplasts move towards the sides of the plant cell to avoid strong light, they accumulate and spread out into a layer on the bottom of the cell at low light to increase the light absorption efficiency. Although the motion of chloroplasts has been studied for over a century, the collective organelle-motion leading to light adapting self-organized structures remains elusive. Here we study the active motion of chloroplasts under dim light conditions, leading to an accumulation in a densely packed quasi-2D layer. We observe burst-like re-arrangements and show that these dynamics resemble colloidal systems close to the glass transition by tracking individual chloroplasts. Furthermore, we provide a minimal mathematical model to uncover relevant system parameters controlling the stability of the dense configuration of chloroplasts. Our study suggests that the meta-stable caging close to the glass-transition in the chloroplast mono-layer serves a physiological relevance. Chloroplasts remain in a spread-out configuration to increase the light uptake, but can easily fluidize when the activity is increased to efficiently re-arrange the structure towards an avoidance state. Our research opens new questions about the role that dynamical phase transitions could play in self-organized intracellular responses of plant cells towards environmental cues.