Measurement of static light scattering of bio-particles

TL;DR

This study employs experimental, theoretical, and simulation methods to analyze static light scattering of bio-particles, specifically Pseudomonas aeruginosa and Mycobacterium smegmatis, to understand their morphological properties.

Contribution

It introduces a novel combined approach using experimental setup, T-matrix theory, and Monte Carlo simulations for bio-particle light scattering analysis.

Findings

Experimental results align with theoretical and simulated data.

The method can distinguish morphological differences of bio-particles.

The approach enhances understanding of bio-particle properties through light scattering.

Abstract

Light scattering by small particles is one of the most prevailing and non-invasive technique for examining the properties of particulate systems chiefly of biological origin. The sub-micron particles including the bio-particles were so chosen because of their importance in biology and biomedical sciences. Light scattering investigation from homogenous, pathogenic Pseudomonas aeruginosa and non-pathogenic Mycobacterium smegmatis was carried out at two different wavelength of incident light, by using an original designed and fabricated polar and azimuth-dependent light scattering setup. Theoretical scattering plots were generated by using T-matrix approach to validate our analyses. Simulations of light scattering of these particles were also carried out using a novel Monte- Carlo simulation technique. The relation between experimental, theoretical and simulated result is presented in this paper. We have studied Pseudomonas aeruginosa and Mycobacterium smegmatis, and attempted for prospect of observing its morphological property by using light scattering tool.