Nonlinear master relation in microscopic mechanical response of semiflexible biopolymer networks

TL;DR

This study investigates the nonlinear microscopic mechanical response of semiflexible biopolymer networks, revealing a universal stiffening behavior through microrheology experiments and a master curve collapse, enhancing understanding of cell mechanics.

Contribution

It introduces a high-bandwidth microrheology method to uncover a universal nonlinear response in biopolymer networks, linking microscopic mechanics to cellular functions.

Findings

Observed affine elastic response with stiffening upon local forcing

Discovered a collapse onto a single master curve after scaling behaviors

Provided a theoretical justification for the universal response

Abstract

A network of semiflexible biopolymers, known as the cytoskeleton, and molecular motors play fundamental mechanical roles in cellular activities. The cytoskeletal response to forces generated by molecular motors is profoundly linked to physiological processes. However, owing to the highly nonlinear mechanical properties, the cytoskeletal response on the microscopic level is largely elusive. The aim of this study is to investigate the microscopic mechanical response of semiflexible biopolymer networks by conducting microrheology (MR) experiments. Micrometer-sized colloidal particles, embedded in semiflexible biopolymer networks, were forced beyond the linear regime at a variety of conditions by using feedback-controlled optical trapping. This high-bandwidth MR technology revealed an affine elastic response, which showed stiffening upon local forcing. After scaling the stiffening behaviors, with parameters describing semi-flexible networks, a collapse onto a single master curve was observed. The physics underlying the general microscopic response is presented to justify the collapse, and its potentials/implications to elucidate cell mechanics is discussed.