Direct measurement of the aerotactic response in a bacterial suspension

TL;DR

This study measures how motile bacteria respond to oxygen gradients by tracking their movement and local oxygen levels, revealing a power-law dependence of aerotactic velocity on oxygen concentration.

Contribution

It combines simultaneous bacteria tracking and oxygen measurement to quantify the aerotactic response, confirming a c^{-2} dependence consistent with receptor-based models.

Findings

Aerotactic response ^{-2} in bacteria.

Consistent results from population and single-cell analyses.

Experimental validation of theoretical models.

Abstract

Aerotaxis is the ability of motile cells to navigate toward oxygen. A key question is the dependence of the aerotactic velocity with the local oxygen concentration c. Here we combine simultaneous bacteria tracking and local oxygen concentration measurements using Ruthenium encapsulated in micelles to characterize the aerotactic response of Burkholderia contaminans, a motile bacterium ubiquitous in the environment. In our experiments, an oxygen gradient is produced by the bacterial respiration in a sealed glass capillary permeable to oxygen at one end, producing a bacterial band traveling toward the oxygen source. We compute the aerotactic response \chi(c) both at the population scale, from the drift velocity in the bacterial band, and at the bacterial scale, from the angular modulation of the run times. Both methods are consistent with a power-law \chi \propto c^{-2}, in good agreement with existing models based on the biochemistry of bacterial membrane receptors.