Yield Stress and Compliance in Active Cell Monolayers

TL;DR

This study uses a multi-phase field model to explore how active cell monolayers transition between solid-like and fluid-like states, revealing how tissue deformability influences yield stress and compliance during microrheology.

Contribution

It introduces a computational model to analyze the rheological behavior of active cell monolayers, highlighting the impact of cell deformability on tissue mechanics and probe motion.

Findings

Solid tissues exhibit yield-stress behavior with a threshold force for probe motion.

High deformability tissues respond smoothly to perturbations, showing amorphous and compliant behavior.

Low deformability tissues are more ordered, stiffer, and show discontinuous probe motion onset.

Abstract

The rheology of biological tissue plays an important role in many processes, from organ formation to cancer invasion. Here, we use a multi-phase field model of motile cells to simulate active microrheology within a tissue monolayer. When unperturbed, the tissue exhibits a transition between a solid-like state and a fluid-like state tuned by cell motility and deformability - the ratio of the energetic costs of steric cell-cell repulsion and cell surface tension. When perturbed, solid tissues exhibit yield-stress behavior, with a threshold force for the onset of motion of a probe particle that vanishes upon approaching the solid-to-liquid transition. This onset of motion is qualitatively different in the low and high deformability regimes. At high deformability, the tissue is amorphous when solid, it responds compliantly to deformations, and the probe transition to motion is smooth. At low deformability, the monolayer is more ordered translationally and stiffer, and the onset of motion appears discontinuous. Our results suggest that cellular or nanoparticle transport in different types of tissues can be fundamentally different, and point to ways in which it can be controlled.