The Biosensor based on electrochemical dynamics of fermentation in yeast Saccharomyces Cerevisiae

TL;DR

This paper presents a novel yeast-based electrochemical biosensor that uses fermentation dynamics measured by pressure sensing and impedance spectroscopy for environmental water quality monitoring, offering a low-cost and non-toxic alternative to bacterial bioluminescence methods.

Contribution

It introduces a new biosensing approach combining pressure sensing and EIS to analyze yeast fermentation, providing more detailed ionic information and field applicability.

Findings

EIS yields more detailed ionic metabolic information.

The method is sensitive to environmental stressors and water treatments.

It offers a low-cost, non-toxic alternative for water quality assessment.

Abstract

The zymase activity of the yeast Saccharomyces Cerevisiae is sensitive to environmental parameters and is therefore used as a microbiological sensor for water quality assessment, ecotoxicological characterization or environmental monitoring. Comparing to bacterial bioluminescence approach, this method has no toxicity, excludes usage of genetically modified microorganisms, and enables low-cost express analysis. This work focuses on measuring the yeast fermentation dynamics based on multichannel pressure sensing and electrochemical impedance spectroscopy (EIS). Measurement results are compared with each other in terms of accuracy, reproducibility and ease of use in the field conditions. It has been shown that EIS provides more information about ionic dynamics of metabolic processes and requires less complex measurements. The conducted experiments demonstrated the sensitivity of this approach for assessing biophotonic phenomena, non-chemical water treatments and impact of environmental stressors.