Effect of In-vivo Heat Challenge on Physiological Parameters and Function of Peripheral Blood Mononuclear Cells in Immune Phenotyped Dairy Cattle

TL;DR

This study investigates how immune phenotyping in dairy cattle correlates with physiological and cellular responses to in-vivo heat stress, revealing that high immune responders exhibit greater heat tolerance and cellular resilience.

Contribution

It provides new insights into the relationship between immune response phenotypes and heat stress resilience in dairy cattle under in-vivo conditions.

Findings

High responders show increased heat shock protein 70 levels.

High responders have greater cell proliferation post-heat challenge.

Average and low responders exhibit higher respiration rates at high temperature humidity index.

Abstract

The frequency of heat waves are increasing due to climate change, which leads to an increase in the occurrence of heat stress in dairy cattle. Previous studies have shown that dairy cattle identified as high immune responders have a reduced incidence of disease and improved vaccine response compared to average and low responders. Additionally, it has been observed that when cells from immune phenotyped cattle are exposed to in-vitro heat challenge, high immune responders exhibit increased heat tolerance compared to average and low responders. Therefore, the objective of this study was to evaluate physiological parameters and the function of blood mononuclear cells in immune phenotyped dairy cattle exposed to in-vivo heat challenge. A total of 24 immune phenotyped lactating dairy cattle (8 high, 8 average and 8 low) were housed in the tie-stall area of the barn and exposed to an in-vivo heat challenge for 4 hours on 2 subsequent days. Blood samples were taken both pre- and post-challenge and respiration rates and rectal temperatures were recorded. Temperature and humidity measurements were taken in correspondence with all respiration rate and rectal temperature measurements to calculate the temperature humidity index. Blood mononuclear cells were isolated from blood collected pre and post challenge and the concentration of heat shock protein 70 and cell proliferation were assessed. Results showed that average and low responders had significantly greater respiration rates compared to high responders at a temperature humidity index of 77 and above. High responders had a higher heat shock protein 70 concentration and greater cell proliferation after in-vivo heat challenge compared to average and low responders. These results paralleled those found during in-vitro heat challenge confirming that high responders may be more resilient to heat stress compared average and low responders.