Evaluation of the Feasibility of Phosphorene for Electronic DNA Sequencing Using Density Functional Theory Calculations

TL;DR

This study uses density functional theory to evaluate phosphorene's potential for electronic DNA sequencing, showing it has advantages over graphene in base discrimination and interaction properties.

Contribution

First-principles computational analysis demonstrating phosphorene's superior properties over graphene for DNA sequencing applications.

Findings

Lower binding energies of DNA bases on phosphorene

Significant energy gap modulations with DNA bases in phosphorene

Phosphorene shows promise as an alternative to graphene for sequencing

Abstract

Electronic DNA sequencing using two-dimensional (2D) materials such as graphene has recently emerged as the next-generation of DNA sequencing technology. Owing to its commercial availability and remarkable physical and conductive properties, graphene has been widely investigated for DNA sequencing by several theoretical and experimental groups. Despite this progress, sequencing using graphene remains a major challenge. This is due to the hydrophobic nature of graphene, which causes DNA bases to stick to its surface via strong {\pi}-{\pi} interactions, reducing translocation speed and increasing error rates. To circumvent this challenge, the scientific community has turned its attention to other 2D materials beyond graphene. One such material is phosphorene. In this article, we performed first-principle computational studies using density functional theory (DFT) to evaluate the ability of phosphorene to distinguish individual DNA bases using two detection principles, namely, nanopore and nanoribbon modalities. We observe that binding energies of DNA bases are lower in phosphorene compared to graphene. The energy gap modulations due to interaction with DNA bases are very significant in phosphorene compared to graphene. Our studies show that phosphorene is superior to graphene, and hence a promising alternative for electronic DNA sequencing.