Protein Enrichment by Flotation: Experiment and Modeling

TL;DR

This study combines experimental and modeling approaches to analyze protein flotation, demonstrating how foam stability and bubble dynamics influence protein recovery and purity, with a model that predicts outcomes based on experimental inputs.

Contribution

The paper introduces a unsteady state simulation model for protein flotation that accurately predicts liquid recovery and protein concentration without tuning parameters.

Findings

Foam coalescence accelerates liquid drainage and improves protein enrichment.

The model accurately reproduces flotation results within a reasonable error margin.

Experimental inputs suffice for the model, enabling potential optimization and scaling.

Abstract

Protein flotation is a process in which protein molecules are enriched by adsorption at rising bubbles. The bubbles then form a foam above the solution, where the liquid drains down and the dried foam, which is concentrated in protein, is extracted. Here, the recovery rate and purity of the extract depend on foam stability, surface coverage, bubble size, gas flow rate, etc. In this work, we performed flotation experiments using bovine serum albumin (BSA). In addition, an unsteady state simulation of the protein flotation process was carried out by numerically solving the liquid drainage equation in the foam. Thereby, the extracted liquid volume and protein concentration at the outlet were calculated with time. Required quantities such as foam stability, interface coverage or bubble size distribution were measured in corresponding experiments and were fed into the model. The experiments showed that the foam coalescence accelerates the liquid drainage leading to dryer extract and higher protein enrichment. The modeling also reproduced the liquid recovery and extract concentration of the flotation tests within a reasonable error range. The modeling solely relies on experimental inputs and does not require any tuning parameters. It can be further used for optimization or up-scaling of protein flotation.