Combining cortical voltage imaging and hippocampal electrophysiology for investigating global, multi-time scale activity interactions in the brain

TL;DR

This paper discusses combining cortical voltage imaging with hippocampal electrophysiology in awake mice to study multi-scale brain activity interactions, advancing neural circuit analysis techniques.

Contribution

It introduces a novel combined methodology of voltage imaging and electrophysiology for investigating brain activity across multiple scales.

Findings

Enables simultaneous cortical and hippocampal activity recording

Facilitates analysis of multi-time scale neural interactions

Applicable to awake, behaving mice

Abstract

A new generation of molecular tools for analyzing neural activity has been contributing to elucidate classical open questions in neuroscience. With enhanced GEVIs and advanced optical imaging techniques, voltage-imaging technologies have been increasingly used to observe the dynamics of large circuits. In this chapter, we describe how to combine cortical wide-field voltage imaging with hippocampal electrophysiology in awake behaving mice. Furthermore, we highlight how this method can be useful for different possible investigations.