Rheological dynamics of active Myxococcus xanthus populations during development

TL;DR

This study investigates the mechanical properties and developmental dynamics of Myxococcus xanthus fruiting bodies, revealing two distinct relaxation times and a transition from fluid-like to elastic structures during maturation.

Contribution

The paper provides the first rheological characterization of fruiting body development, linking cellular motility to viscoelastic properties and maturation processes.

Findings

Relaxation occurs on two time scales: ~1 s and ~100 s.

Cell motility drives early droplet flow, ceasing as spores form.

Modulus increases dramatically during maturation, forming resilient structures.

Abstract

The bacterium Myxoccocus xanthus produces multicellular protective droplets called fruiting bodies when starved. These structures form initially through the active dewetting of cells into surface-bound droplets, where substantial flows of the material are needed as the fruiting bodies grow and become round. These dynamics are followed by a primitive developmental process in which the fluid-like droplets of motile cells mature into mechanically-resilient mounds of non-motile spores that can resist significant mechanical perturbation from the external environment. To date, the mechanical properties of fruiting bodies and the changes in cellular behavior that lead to maturation have not been studied. We use atomic force microscopy to probe the rheology of droplets throughout their development and find that relaxation occurs on two time scales, $\sim$1~s and $\sim$100~s. We use a two-element Maxwell-Wiechert model to quantify the viscoelastic relaxation and find that at early developmental times, cellular motility is responsible for the flow of the material but that this flow ceases when cells stop moving and become nonmotile spores. Later in development there is a dramatic increase in the modulus of the droplet as cells sporulate and the fruiting body matures, resulting in a mostly elastic structure that can protect spores from harsh environmental insult.