To cross or not to cross: collective swimming of Escherichia coli under two-dimensional confinement

TL;DR

This study investigates how confinement in two-dimensional environments affects the collective swimming behavior of Escherichia coli, revealing a transition from nematic order to clustering with short-range polar order due to changes in chamber height.

Contribution

It demonstrates how slight variations in confinement geometry can drastically alter bacterial collective phases and provides microscopic insights into these phenomena.

Findings

Long-range nematic order occurs when bacteria can cross during encounters.

Decreasing chamber height prevents crossing, leading to bacterial clustering.

The study offers a method to control collective phase symmetry through confinement.

Abstract

Bacteria in bulk fluids swim collectively and display fascinating emergent dynamics. Although bacterial collective swimming in three-dimensional (3D) geometries has been well studied, its counterpart in confined two-dimensional (2D) geometries relevant to natural habitats of bacteria is still poorly understood. Here, through carefully designed experiments on Escherichia coli in Hele-Shaw chambers, we show that a small change in the degree of confinement leads to a drastic change in bacterial collective swimming. While long-range nematic order emerges for bacteria that can cross during encounters, a slight decrease of the chamber height prevents the crossing, leading to the formation of bacterial clusters with short-range polar order. By tracking the swimming kinetics of individual bacteria, we reveal the microscopic origins of the two collective phases. Our study provides important insights into bacterial collective swimming under confinement and demonstrates a convenient way to control the emergent symmetry of collective phases.