Effect of He Self-organized pattern plasma-activated media with different conductivity on cancer cells

TL;DR

This study investigates how self-organized pattern (SOP) plasma discharge modes in helium influence reactive species in plasma-activated media with different conductivities, affecting cancer cell viability and providing insights into plasma-based cancer therapy mechanisms.

Contribution

It demonstrates the ability to control reactive species in plasma-activated media using SOP discharge modes and compares electrode effects on cancer cell cytotoxicity.

Findings

SOP discharge modes modulate ROS and RNS levels in plasma-activated media.

Reactive species concentrations influence cancer cell death.

Electrode material impacts cell viability after plasma treatment.

Abstract

The self-organized pattern (SOP) phenomenon is prevalent in plasma, while knowledge about SOP discharge affecting reactive species generated plasma-activated media (PAM) for cancer therapy is poorly documented. The aim of this study focused on the effect of SOP discharge modes on reactive oxygen and nitrogen species (ROS, RNS) in He SOP plasma-activated media with different conductivity (saline solution and deionized (DI) water), and employed them to breast cancer MDA-MB-231 and pancreatic BxPC-3 cancer cells. Optical emission spectrum and Fluorimetric analysis were used to identify and quantify ROS and RNS generated in He SOP plasma-activated saline solution and DI water. Furthermore, He SOP plasma discharge modes are capable of efficiently controlling the ROS and RNS concentration in the plasma-activated saline solution and DI water, which contribute to the cytotoxic effect. On the other hand, stainless steel and copper were used as a lower electrode to compare their effect on cell viability. Taken together, our findings provide insight into potential mechanisms involved in cell death after treatment with He SOP plasma-activated media.